44ANTI MICROBIAL EVALUATION OF POLYHERBAL EXTRACT IN THE TREATMENT OF PERIODONTITISTo formulate and evaluate polyherbal extract, prepared by combined extracts of Psidium guajava, Emblica officinalis, Terminalia bellerica L and Syzygium aromaticum for treatment of Periodontitis. The formulation containing herbs viz., the leaves of Psidium guajava (Myrtaecae), fruits of Emblica officinalis L. (Euphorbiaceae), leaves of Terminalia bellerica L. (Combretaceae), dried flower buds of Syzygium aromaticum (Myrtaceae),The Antimicrobial efficacy of the formulated Polyherbal extract was tested on Streptococcus mutans, Escherichia coli, Staphylococcus aureus and Proteus vulgaris by Dip well technique. Dip well method showed that the polyherbal extract has good antimicrobial activity when compared with the standard Antibiotic. The present study revealed that the polyherbal extract exhibited comparatively better activity than standard.fytopharmshama@gmail.comResearch412014Periodontitis,Antimicrobial,Polyherbal extract,HerbsS. Neelufar Shama,K.R. Prasanna,K. ThamizhvananDepartment of Pharmacognosy, Sree Vidyanikethan College of Pharmacy, Tirupati, Andhra Pradesh, India,Department of Pharmacognosy, Sree Vidyanikethan College of Pharmacy, Tirupati, Andhra Pradesh, India,Department of Biotechnology, Sree Vidyanikethan College of Pharmacy, Tirupati, Andhra Pradesh, India.