61ANTI -INFLAMMATORY ACTIVITY OF VARIOUS FRACTIONS OF METHANOLIC EXTRACT OF PUNICA GRANATUM RIND WITH ITS PHYTOCHEMICAL EVALUATIONObjective: To evaluate anti-inflammatory potential of rind extract of Punica granatum by invitro antiinflammatory model and its phytochemical evaluation. Method: The fractionation of methanolic extract of Punica granatum rind was done using various solvents and evaluated for anti-inflammatory activity using in -vitro human red blood cell (HRBC) membrane stabilization method and its phytochemical study. Results: The in -vitro membrane stabilizing test showed that butanol, n -hexane and aqueous extracts have potent anti-inflammatory activity which were 42.66%, 14.1% and 28% protection, respectively as compared to Standard drug. The potency of the rind extracts of PG were compared with standard diclofenac in stabilization test and which showed 81.76% protection in in -vitro HRBC membrane stabilization method. The extract showed the dose dependent anti-inflammatory activity. Conclusions: The present investigation has confirmed the anti-inflammatory activity of fractions of methano lic extract of Punica granatum rind due to presence of alkaloids, steroids, terpinoids for the first time and provided the pharmacological evidence in the favour of traditional uses of Punica granatum as an anti-inflammatory agentnainwani.rahul29@gmail.co mReview432014Punica granatum,Anti-inflammatory activit,HRBC membrane stabilization methodRahul Nainwani,Divya singh,Amit1 Gupta,Mohit Batra,Patel HardikDepartment of Pharmacology, Jaipur College of Pharmacy , ISI-15, RIICO Institutional Area, Tonk Road, Sitapura, Jaipur, India,Department of Pharmacology, Jaipur College of Pharmacy , ISI-15, RIICO Institutional Area, Tonk Road, Sitapura, Jaipur, India,Department of Pharmacology, Jaipur College of Pharmacy , ISI-15, RIICO Institutional Area, Tonk Road, Sitapura, Jaipur, India,Department of Pharmaceutics, Mahatma Gandhi College of Pharmaceutical Sciences , Jaipur, Rajasthan 302022, India.,Department of Pharmacology, Jaipur College of Pharmacy , ISI-15, RIICO Institutional Area, Tonk Road, Sitapura, Jaipur, India